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Shodex Gel Permeation Chromatography (GPC) Columns


GPC, using organic solvents, is used primarily for the analysis of polymers.

Separation occurs on the basis of decreasing molecular size, which to a first approximation equates to decreasing molecular weight. No chemical interaction must occur between the sample and the packing material.

A column is defined by its Exclusion Limit. Molecules bigger than this cannot enter the pores and pass unretained through the column. However columns with high exclusion limits offer very limited resolution of species with low molecular weight, so it is normal to require more than one column.

The total volume of the pores is known as the "Inner Volume" of the system, Vi. The largest peaks (at or above the Exclusion Limit) all elute in the Void volume of the system (V0). All peak will elute between V0 and V0+Vi. So it follows that the bigger the pore volume of the system, the better resolution can be achieved, and this is why GPC columns are usually wider and longer than normal HPLC Columns.

To separate a wide range of molecular sizes, there are two standard approaches:

  1. Use a series of 3-4 different columns connected in series, each covering a different molecular weight range. Careful selection of the columns used (eg exclusion limits 400,000 + 70,000 + 5,000) will give a fairly linear calibration curve over the desired range.
  2. Use 3 x Mixed bed columns. Effectively the packings from the three different column are mixed, and packed into the columns so that the samples pass through a continuous bed, and the separation occurs slowly through the columns. This gives a much better linearity and is always recommended where a suitable column is available.

Shodex have a range of columns to suit both of these approaches, and have also developed a third option (see next paragraph), unique to Shodex, which gives even better results. Mixed bed columns are designated with an M and a special version offering a really Linear calibration curve are designated with an L. Both the L and M versions are mixed bed columns, but the L version is a newer development, giveing a more linear calibration curve.

The latest approach has been achieved by developing a packing material with a wide pore-size distribution, so that it can cope with a wide molecular weight range using a single packing material. This gives a totally homogenous packing of the column, and the pore size distribution has been fine-tuned to give a truly linear calibration curve accross a wide range of molecular weights. We have a 17 page Technical Notebook from Shodex describing the use of this new column type, and these are available free of charge. To request a copy please send an email to, giving your name and company address, and we will be pleased to send you one by return.

Column format in GPC. Shodex offer three column formats for GPC:

  1. The standard column size for GPC is 8.0 x 300mm. This is to achieve the large pore volume required, as described above. For most separations, this is the column size to choose.
  2. Shodex also offer a Rapid Analysis format. These columns are 6.0mm id, and 150mm long. Analysis times are faster, because the column volmes are much lower. To combat the inherent reduction in resolution, these columns are offered with a smaller particle size packing.
  3. Then there is the High Resolution Format. These columns are 4.6mm x 250mm. They use a very small particle size, yet are almost as long as a standard column, so with a slower flow rate the resolution is better than either of the other two. The smaller sample size means that a smaller flow cell can be used, thus developing almost 4 x the sensitivity of a standard column system.

Eluent choice for GPC is dependent upon several factors. Firstly, the sample must be soluble in the solvent used. This is not always as obvious as it seems, and often an elevated temperature is required to achieve total solubility. Secondly, the solvent ensures that there is no retention due to any interaction with the column other than that of size exclusion. And thirdly, since detection is usually by refractive index difference, the solvent should have as big a difference in RI from the samples as possible. Where possible, columns should be supplied in the solvent to be used, and hence Shodex GPC columns are offered in a range of solvents. Changing solvent with GPC columns is not as easy as with HPLC, and great care must be taken to follow the prescribed procedure.

Solvent Peak Separation Columns are available with some ranges of Shodex GPC columns. These are small pore size columns which cause the solvent used to dissolve the sample to elute a little after the end of the chromatogram. It also can improve the separation generally in the low molecular weight range. Further details can be found by following this link to the Shodex website:

Guard Columns are used in GPC to prevent any chemical impurities in the polymer sample from binding chemically to the GPC column.

Sample preparation is very important for GPC, especially so for large molecules. Separation is achieved on the basis of molecular size, so it is important that the molecules are allowed to swell and stabilize in the solvent being used as eluent. This make take several hours. Where possible, the eluent should be the sample solvent. Sample concentration is also very important.
Features of columns to note are:
Exclusion Limit
The exclusion limit is the molecular weight at and above which the samples elute with the void volume from the column.
Particle Size
Typically 3-20 μm. The samller the particles the higher the efficiency (sharper the peaks) but the higher the back pressure.
Some GPC columns give sharper peaks than others.
If possible, choose a column which is packed in the solventwith which it is going to be used. This minimises the effects of gel swelling caused by solvent changes, and increases the lifetime of the column.
A "standard" GPC column is 8.0mm x 30cm, but other sizes are available for high speed (6mmid) and for high resolution GPC (4.6mm).
Temp Range
Some solvents are viscous and need to be used at elevated temperatures. Some polymers are very hard to dissolve and require very high temperatures. For the highest temperatures we have special column available which can take up to 210C.
Max Pressure
Some polymers can take more back pressure than others so check the maximum and do not exceed it.
Column Details
GPC Packing
Exclusion limit range
Efficiency (N/m)
Packing Solvent
Particle size (um)

Max. Temp. (C)

Maximum flow rate (ml/min)

Max. Pressure



Styrene Divinylbenzene

1,500 - 200,000,000


3 -18

THF is the most commonly used eluent in GPC, so this is the most widely used range of columns. A wide range of columns is available, ranging from KF-801 (excl.limit 1,500) up to KF-807 (excl limit 200,000,000). KF806 is available as a mixed bed column (suffix M), and 803, 805, 806 and 807 are available as Linear Mixed bed columns, offering a wide range Linear calibration curve. These columns have suffix L. Note that there are three listings in the pricing table, covering the standard 8.0mm columns, the high speed 6mm columns, and the high resolution 4.6mm columns


Styrene Divinylbenzene

1,500 - 200,000,000


6 - 18

Chloroform is the second most commonly used GPC solvent. Mixed bed and Linear columns are available as above, but note that the high speed and high resolution column sizes are not available packed with chloroform as standard. If there is a column spec that you require which is not listed, please ask. Most things are possible!


Styrene Divinylbenzene

2,500 - 200,000,000

Polyethylene Glycol

Dimethyl Formamide
6 - 18

Dimethyl Formamide is suitable for analysis of polar polymers, such as melamine resin, polyacrylonitrile, polyvinylpyrrolidone, polyimide resin. This range includes a mixed bed column, but not the Linear range columns, and is not available as standard in the high speed or high resolution formats.

Styrene Divinylbenzene

2,500- >2,000,000


Hexafluoro Isopropanol
3 - 18

These columns are used exclusively with HFIP, which allows the room-temperature analysis of plastics such polyamides and polyethylene terephthalate. These columns are also available in the rapid analysis format, 6.0mm id.


Styrene Divinylbenzene




This is the new gel with the wide pore size distribution. It gives a Linear calibration curve over a wide range of molecular weights. If applicable, this is definitely the column range to choose! A technical notebook is available on request from Laserchrom.


Styrene Divinylbenzene

70,000 - 200,000,000


13 - 18

For high temperature work, up to 140C, these are the columns to choose. At higher temperatures use the UT range, but note that they offer lower efficiency, so use an HT column if possible.

Styrene Divinylbenzene

20,000 - 200,000,000



Designed for ultra-high temperature GPC. This is used fopr the analysis of very large polymers or insoluble polymers such as polyethylene or polyprpylene. These columns also prevent shear degradation of polymers in the 200,000,000 range. See Shodex technical note here.