Acetonitrile is used as an eluent in reversed phase HPLC. It is an excellent eluent, having low UV absorption, good selectivity (partly because of its dipole moment) and low viscosity.
Two grades of HPLC Acetonitrile are available. Acetonitrile General is usable down to about 220nm, while the Far UV grade is a higher purity, and usable down to nearly 200nm.
If working at low wavelength and one day no peaks are seen, check that the general grade acetonitrile has not be used by accident.
For further details see the Wikipedia page:
Acetonitrile |
|
Acetonitrile Far UV
| Assay |
|
>99.9% |
| Residue |
|
<0.0001% |
| Water |
|
<0.01% |
| UV Transmission |
| 190nm |
|
>10% |
| 192nm |
|
>50% |
| 195nm |
|
>80% |
| 215nm |
|
>95% |
| 220-400nm |
|
>99% |
| Cat No |
Size |
Price |
| LC004W/LC |
2.5l |
£N/A |
|
R11
R20/21/22
R36
S1/2
S16
S36/37 |
Highly flammable
Harmful by inhalation, in contact with skin and if swallowed.
Irritating to eyes.
Keep locked up, and out of the reach of children.
Keep away from sources of ignition. No smoking.
Wear suitable protective clothing and gloves |
Boiling Point
Freezing point
Flash Point
Density
Refractive index
Viscosity |
81.6C
-48C
6C
0.78
1.345
0.36cP |
|