The HPLC Solvent Guide (2002) 2nd Edition

Paul Sadek (Analytical Consulting Laboratories, Michigan, USA)

0-471-41138-8 hardback

693 pages

Laserchrom Review : This is a particularly useful book for labs involved with HPLC Method Development. Only the first three chapters deal with actual solvent properties, such as viscosity, UV cut-off, miscibility etc, solvent optimisation and method development. The remaining chapters (over 575 pages) cover examples of solvent systems used for almost every class of analyte experienced by the author. I personally have found this to be really useful on numerous occasions, and almost everything I have looked up has been in there somewhere! Clearly you get 69,000 answers to any given problem you need on the internet, but this book is a really useful starting point, and may even solve a problem before any method development is attempted.
One useful extra is that the reasons for the various eluent choices are explained. Hence if an eluent contains an unusual additive or pH buffer, or an unexpected wavelength is chosen, the reasons are given, and useful suggestions made.
This is not at all a theoretical book. But it is clear from the start that the author has massive practical experience, and his information is your information for £59.50.

Publishers Summary :High Performance Liquid Chromatography (HPLC) is a procedure for characterizing mixtures of chemical substances from a wide range of matrices, identification and quantitative measurements. Solvent selection is perhaps the most commonly overlooked parameter in HPLC - even the most experienced analytical chemist tends to select one of three familiar solvents. The HPLC Solvent Guide provides detailed coverage of all HPLC Solvents commonly used in a wide range of seperations.

Building on the success of the first edition, the Second Edition discusses method optimisation techniques (triangulation, window diagrams), presents the concept of method evaluation (including functional defenitions and general guidelines for validatiuon protocols), and analyses syustem suitability parameters. More than 16,000 abstracted references are reviewed, most from citattions postdating the first edition and with increased emphasis being placed on natural products, botanicals and herbals.

This new revision offers a detailed and expanded presentation reviewing all commonly used solvent classes ad chemical and physical properties; provides easy references for solvent class, field of application and specific analyte class; describes each seperation fully, listing sample matrix, analystes seperated and quantified, chromatographic parameters used and abbreviated results; includes popular "how to" and "how not to" methodology; focuses on practical applications and use of method optimisation processes.

Professionals in the pharmaceutical and biotechnological industries and university researchers and faculty members will find The HPLC Solvent Guide an indispensable resource.

 Price : £59:50