Laserchrom HPLC Training Courses
HPLC Method Development
 
For dates, prices etc, please follow the link below to our new training centre: http://www.hplccourses.com/StuartJonesHPLCAcademyHPLCMethodDevelopmentCourse.htm
 
Course Contents
Over 3 days the course covers the theoretical principles of method development including choice of equipment, solvent, column, detection method and a more advanced look at data handling. This course concludes with an assessment and presentation of certificates.

HPLC in terms of :

  • Selectivity
  • Relative Retention Time
  • Efficiency
  • Resolution
  • Asymmetry
  • Solvophobic Theory

Requirements of an HPLC method

Objectives of an HPLC method

  • Resolution
  • Speed
  • Accuracy/Precision
  • Robustness
  • Sensitivity

Establishing a starting point for method development

Running an initial gradient

  • What can be learned from the result

Choice of Column

Choice of solvent

%B Optimisation

Temperature Optimisation

Choice of buffer

Optimising buffer concentration

Choice of Isocratic or gradient elution

Effect of changing gradient profile and run time

Setting up integration conditions

Setting up an appropriate calibration

  • Area %
  • Internal Standards
  • External Standards
  • Calibration Range
  • Limit of Detection (LOD)
  • Limit of Quantitation (LOQ)

Analysis of errors and how to minimise them

Changing detector to increase sensitivity

Establishing confidence in peak identification

Sample preparation

IQ/OQ/PQ

Standard operating procedures

Method Validation

Risk assessment

Case Studies

Course assessment




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