| Course Content |
|
1)
|
Overview
of HPLC equipment |
|
2)
|
Correct
installation of an HPLC system, including all fluid and electrical
connections |
|
3)
|
Condition
required for dynamic equilibration |
| 4) |
Routine
Maintenance |
| |
- Replacement of pistons
- Replacement of piston seals
- Replacement of check valves
- Replacement of lamps
|
|
5)
|
A wide
range of symptoms are covered including: |
| |
- Retention times increasing and
decreasing
- Retention times unreproducible
- Unreproducible quantitation
- Broad peaks
- Ghost peaks
- Shoulders on peaks
- Tailing peaks
- Double peaks
- No peaks
- Negative peaks
- Flat topped peaks
- Drifting baseline
- Noisy baseline
- Spiking baseline
- Baseline shifts
- Long term noise
- Stepping baseline
- High back pressure
- Fluctuating back pressure
- Low back pressure
- Rising back pressure
|
|
6)
|
Corrective
Measures |
|
- Stopping leaks
- Replacing flow cells
- Testing lamp energy
- Making new fittings
- Rebuilding injection valves
- Replacing connecting tubing
|
|
7)
|
Practical
Troubleshooting |
| 8) |
Course
Assesment |
