Gel Permeation Chromatography (GPC) usually refers to the separation of organic polymers by size exclusion. Gel Filtration Chromatography (GFC) is used to refer to separation of biological polymers such as nucleotides, peptides, proteins and polysaccharides in aqueous solution using the SEC principles. This course currently covers both.

Day 1

Principles of GPC

Separation Principles

Column exclusion limit

Void volume and Pore volume of the system

Separation of a wide molecular weight range

Using 3 or 4 columns of different types in series

Using 3 or 4 mixed bed columns

Using 3 or 4 columns of mixed pore-size gel

Column formats in GPC

Standard columns 8.0 x 300mm

Rapid Analysis Format 6.0 x 150mm

High Resolution Format 4.6 x 250mm

Solvent Peak Separation Columns

Guard Columns

Eluent Choice

Sample solubility

Prevention of Chmical Interaction with the column

Refractive index

Sample preparation

Sample Concentration and dilution

Swelling of large molecules in solution

Molecular weight calibration

Calibration range

Calibration standards

Solvent dependence

Detection for GPC

UV

Refractive index

Evaporative Light Scattering Detectors

GPC Data Systems

Day 2

Lab Practical