When ordering HPLC tubing it
is important to choose the correct material (steel, PEEK, PTFE
or tygon) the outer diameter (1/16" or 1/8") and the
internal diameter (0.010", 0.020", 0.030", 0.04"
Use high pressure tubing from
the pump through to the detector.
To prevent band broading, use 0.010" i.d. tubing from the
injector to the column and from the column to the detector and
keep each length as short as possible. Use Tygon tubing for piston